“One-Pot” Process for Preparation of Peptides and Protein-Silica Hybrid Capillary Monolith

整体柱是近年来发展的一种新型色谱分离介质，具有制备简单、通透性好、传质速率快、无需柱塞等优点，在分离和富集领域得到了较大的发展和应用。整体柱可以分为有机聚合物整体柱、硅胶整体柱和有机-无机杂化硅胶整体柱三大类。有机聚合物整体柱制备过程简单、耐酸碱、可选用的单体种类多、适合分离生物大分子，但整体柱基质易在有机溶剂中溶胀。硅胶整体柱机械强度高，对小分子的分离效率高，但制备过程复杂，pH适用范围小（pH2-8）。有机-无机杂化硅胶整体柱则克服了聚合物和硅胶两种整体柱的缺点，耐酸碱，机械性能好。但由于有机硅氧烷试剂种类的限制，使得以溶胶-凝胶法将有机功能单体键合到硅胶基质上以制备各种不同功能的整体柱受...Monolithic column has been developed as a novel state-of-the-art stationary phase with the advantages of facile preparation, high permeability, fast mass transfer and fritless design. It thus attracts more and more scientists to working in the fields of separation science and sample enrichment. In general, monolithic columns can be classified into three kinds: polymer-based monolith, silica-based ...学位：理学硕士院系专业：化学化工学院_分析化学学号：2052011115167